Girlguiding leaders in Birstall, Leicestershire, fear they will have nowhere to meet unless funding is found for urgent repairs to their headquarters.
The building in Johnson Road is used by Rainbows, Brownies, Guides and Rangers.
Brownie leader Marie Upton, 52, said the building needed a new floor and a new roof, which would cost "thousands of pounds".
She said the Guides were grateful for being named the main beneficiary of the village's charity Duck Race on Monday.
Ms Upton said: "We're very privileged that the White Horse have made us main beneficiaries of the Duck Race this year.
"At the minute we've got an infestation of woodworm [in the floor] which was treated once but can't be treated again."
She said they were hoping to use the funds from the duck race to get the floor fixed over the summer, which will mean the building can "go on for generations to come".
